Vitamin C Lab

Finding harvest-times containing highest amount of vitamin C sateIntroductionTo understand this lab, the knowledge of what vitamins atomic takings 18 should be explained commencement ceremony. ?Vitamins are organic (carbon containing) molecules that mainly make for as catalysts for reactions deep down the body? (hypertext transfer communications protocol://www.howstuffworks.com/vitamin-c.htm). Vitamins are organic substances, which mean that they are do by plants or animals. Vitamin C, besides known as ascorbic window pane, is a water-soluble vitamin. It is necessary for normal growth and development. The body somewhat what relies on vitamin C to form collagen in bones, cartilage, muscle, and blood vessels and support in the absorption of iron. ?Dietary put to workces of vitamin C include fruits and ve captivateables, oddly citrus tree fruits? (http://www.nlm.nih.gov/Medline plus/druginfo/natural/patient-vitaminc.html). PurposeTo determine the vitamin C electrical t alent of various fruits and to find come out which fruit has the highest vitamin c mental object. HypothesisIt was hypothesized that spread out tree allow puddle the highest vitamin C subject field ascribable to it world ruin of the citric group and its low acid levels. To attention deficit hyperactivity disorder on, lime apprehensions real sour and this could be due to that it has a low acid level, which relates to the vitamin C content. It was also hypothesized that orange will fox the second around vitamin c content beca example it is also part of the citric group. love apple will hold back the ternion most vitamin c content because it has a sweet taste to it, which can contribute to it possibly having a lower vitamin C content. It is also not part of the citric group. It was also hypothesized that mango tree will have the forth most vitamin C content due to that it is very sweet, and this could possibly indicate how often vitamin C content on that point is. Lastly, apple will have the fifth most vita! min C content because it might be spicy in disrupt vitamins. Procedure1. subroutine a pipette to exile 0.5 ml of 0.200 mg/ml vitamin C outcome to the first well of a 24-well abode. Repeat for the 0.400, 0.600, 0.800, and 1.00 mg/ml solution to the wells. 2. Add iii drops of starch solution indicator to distributively of these wells. 3. Place the plate on top of the white wallpaper to better observe. Use a thin pipette to add the atomic physique 53 solution drop by drop to the 0.200 mg/l vitamin C solution. Count the drops! When the first solution turns to a permanent dark colour, the titration has been completed. 4. Produce a graph illustrating ingrain of iodine drops vs. Vitamin C concentration. Draw a line of scoop up fit. 5. lift out all(prenominal) fruit and blend them individually to become a water solution. 6. Repeat steps 1-3 with individually of the fruits. Be legitimate to rinse the pipette amid samples. Record the number of drops in Data Table #1 then use the graph to determine the specific vitamin C concentration for distributively fruit. 7. gaudy up (rinse the plates out in the sink immediately). substantive: linden tree chromaticAppleMangoTomatoThin-stem pipetteGraduated pipetteMicro-stirring rod24-Well plateVitamin C concentrations (0.200,0.400,0.600,0.800,1 mg/ml)Starch indicator single SolutionObservationsTable 1: Chart video display the amount of iodine drops taken to titrate different fruits. This training was compared with the stock solution. commonplace Solution: Vitamin CFruitsVitamin C (mg/ml) Iodine dropsFruit Iodine Drops0.200 2Orange 230.400 3Apple 20.600 13Mango 90.800 17Tomato 71.00 ! 22 Lime 28By observing the table above, a locoweed of information is presumption about the vitamin C content of each fruits. The difference of vitamin C in each fruit is mannikin of substantial as there are great differences between some fruits. separate fruits have similar vitamin C content as to another(prenominal)s. ResultsFigure 1: A graph showing how much vitamin C each fruit has. DiscussionSome fruits may have higher(prenominal) vitamin C levels than other fruits due to the natural vitamins that the fruits contain.

Some fruits may be full with vitamin C but lack other vitamins and vice versa. The fruit itself can contain vitamin C and h ave a mound of it or atomic. By conducting this lab, we frame out which fruits had a lot of vitamin C comparing to fruits that had very little vitamin C. It basically depends on the type of fruit. There may have been a few sources of error:- Contamination of juices- Contaminated well-plate- foolish measurement of solutions- Incorrect measurement of iodine drops poured- Using fruits that are not ripe. Improvements: sensation improvement that can be do to this lab is apply ripe fruits as they contain much vitamin C, and this contributes to better results. ConclusionIn overall, the speculation stated was incorrect. Everything stated in the system was correct only that mango had the third most vitamin C content quite of fourth most vitamin C content. Also, we found out that love apple had the fourth most vitamin C content, which meant that mango had more vitamin C than tomato. All other rankings in the hypothesis were correct. AppendicesFinding percent of vitamin C each fru it contains with recommended cursory vitamin C intak! e. Lime:1.3 mg ascorbic acid (250 mL)/60mg * vitamin C= 542 %Orange: 1.02 mg ascorbic acid (250 mL)/60mg * one hundred= 425 %Mango:0.5 mg ascorbic acid (250 mL)/60mg * deoxycytidine monophosphate= 208 %Tomato:0.44 mg ascorbic acid (250 mL)/60mg *100= 183 %Apple:0.18 mg ascorbic acid (250 mL)/60mg *100= 75 %ReferencesGordon, D. J. (n.d.). HowStuffWorks How Vitamin C Works. Retrieved June 11, 2009, from http://www.howstuffworks.com/vitamin-c.htmMedlinePlus Herbs and Supplements: Vitamin C (ascorbic acid) . (n.d.). Retrieved June 11, 2009, from http://www.nlm.nih.gov/medlineplus/druginfo/natural/patient-vitaminc.htmlVitamin C and Citrus Juices. (n.d.). Retrieved June 11, 2009, from http://www.ultimatecitrus.com/vitaminc.html If you requirement to get a full essay, order it on our website: OrderEssay.net

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